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Functional screen of inflammatory bowel disease genes reveals key epithelial functions.

Jessy Carol NtunzwenimanaGabrielle BoucherJean PaquetteHugues GosselinAzadeh AlikashaniNicolas MorinClaudine BeauchampLouise ThauvetteMarie-Ève RivardFrédérique DupuisSonia DeschênesSylvain FoisyFrédéric LatourGeneviève LavalléeMark J DalyRamnik J Xaviernull nullGuy CharronPhilippe GoyetteJohn D Rioux
Published in: Genome medicine (2021)
This functional screen, based on expressing IBD genes within an appropriate cellular context, in this instance intestinal epithelial cells, resulted in changes to the cell's transcriptome that are relevant to their endogenous biological function(s). This not only helped in identifying likely causal genes within genetic loci but also provided insight into their biological functions. Furthermore, this work has highlighted the central role of intestinal epithelial cells in IBD pathophysiology, providing a scientific rationale for a drug development strategy that targets epithelial functions in addition to the current therapies targeting immune functions.
Keyphrases
  • genome wide
  • dna methylation
  • single cell
  • high throughput
  • bioinformatics analysis
  • clinical trial
  • gene expression
  • ulcerative colitis
  • genome wide analysis
  • drug delivery
  • mesenchymal stem cells