Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.

Michael Boettcher Sergio CovarrubiasAnne BitonJames BlauHaopeng WangNoah ZaitlenMichael T McManus

Published in: BMC genomics (2019)

Using our approach, we illustrate how heterogeneity in growth kinetics and treatment response of clonal cell lines impairs the results of pooled genetic screens.

Keyphrases

genome wide
single cell
high throughput
dna methylation
copy number
phase iii
gene expression