A genetically engineered "reinforced concrete" scaffold regulates the N2 neutrophil innate immune cascade to repair bone defects.
Wenxiao ShiYu FengJincheng TangYichang XuWang WeiLichen ZhangXinzhao JiangZhouye DingKun XiLiang ChenYong GuPublished in: Advanced healthcare materials (2024)
The innate immune response is crucial to the inflammation, but how neutrophils and macrophages act in bone repair and tissue engineering treatment strategies await clarification. In this study, we found N2 neutrophils release stronger "eat me" signals to induce macrophage phagocytosis which then polarize into the M2c anti-inflammatory phenotype. This is beneficial for bone repair. Guided by this biological mechanism, we filled a mesoporous bioactive glass scaffold (MBG) with HAMA hydrogel loaded with TGFβ1 adenovirus (Ad@H), constructing a genetically engineered composite scaffold (Ad@H/M). The scaffold not only has good hydrophilicity and biocompatibility, but also provides mechanical stress support for bone repair. Adenovirus infection quickly induces N2 neutrophils, upregulating NF-κB and MAPK signaling pathways through Toll-like receptor 4 (TLR4) to promote the inflammatory response and macrophage phagocytosis. Macrophages perform phagocytosis and polarize towards the M2 phenotype, mediating the inflammatory response by inhibiting the PI3K-AKT-NF-κB pathway, maintaining homeostasis of the osteogenic microenvironment. The role of Ad@H/M scaffold in regulating early inflammation and promoting long-term bone regeneration was further validated in vivo. In brief, this study focuses on the cascade of reactions between neutrophils and macrophage subtypes, and reports a composite scaffold that coordinates the innate immune response to promote bone repair. This article is protected by copyright. All rights reserved.
Keyphrases
- tissue engineering
- inflammatory response
- toll like receptor
- bone regeneration
- immune response
- signaling pathway
- innate immune
- lps induced
- bone mineral density
- nuclear factor
- oxidative stress
- lipopolysaccharide induced
- pi k akt
- soft tissue
- adipose tissue
- mesenchymal stem cells
- stem cells
- emergency department
- drug delivery
- dendritic cells
- epithelial mesenchymal transition
- anti inflammatory
- cell proliferation
- cancer therapy
- adverse drug
- induced apoptosis