Detection of interaction between an RNA aptamer and its target compound in living human cells using 2D in-cell NMR.
Omar EladlYudai YamaokiKeiko KondoTakashi NagataMasato KatahiraPublished in: Chemical communications (Cambridge, England) (2022)
We introduced an isotopically labeled RNA aptamer for HIV-1 Tat prepared by E. coli transcription into HeLa cells. We successfully recorded the first heteronuclear 2D in-cell NMR spectra, which makes it possible to study the interaction of the RNA aptamer with argininamide in living human cells with higher resolution.
Keyphrases
- gold nanoparticles
- label free
- single cell
- magnetic resonance
- sensitive detection
- cell therapy
- high resolution
- cell cycle arrest
- induced apoptosis
- escherichia coli
- antiretroviral therapy
- hiv infected
- human immunodeficiency virus
- hepatitis c virus
- nucleic acid
- hiv positive
- solid state
- stem cells
- hiv testing
- oxidative stress
- hiv aids
- signaling pathway
- bone marrow
- south africa
- men who have sex with men