Preparation of five high-purity iridoid glycosides from Gardenia jasminoides Eills by molecularly imprinted solid-phase extraction integrated with preparative liquid chromatography.
Lizong ChenRongyu WangLi CuiXiao WangLeilei WangFanyong SongWenhua JiPublished in: Journal of separation science (2018)
Five iridoid glycosides were prepared using molecularly imprinted solid-phase extraction combined with preparative high-performance liquid chromatography. Hydrophilic molecularly imprinted polymers were synthesized using α-1-allyl-2-N-acetyl glucosamine, which introduced an abundance of hydrophilic groups into the polymers. Using molecularly imprinted solid-phase extraction as the sample pretreatment procedure, five iridoid glycosides, gardenoside, geniposide, shanzhiside, geniposidic acid, and genipin-1-O-gentiobioside, were selectively enriched from Gardenia fructus extracts. Preparative high-performance liquid chromatography then provided iridoid glycosides with a purity >98%. The structures were elucidated by using nuclear magnetic resonance spectroscopy, optical rotation and melting point measurements, and mass spectrometry. The results demonstrate that molecularly imprinted solid-phase extraction combined with preparative high-performance liquid chromatography was an efficient, rapid, and economical method for the preparation of bioactive compounds from natural products.
Keyphrases
- solid phase extraction
- molecularly imprinted
- high performance liquid chromatography
- liquid chromatography
- mass spectrometry
- tandem mass spectrometry
- liquid chromatography tandem mass spectrometry
- simultaneous determination
- high resolution mass spectrometry
- high resolution
- ultra high performance liquid chromatography
- gas chromatography mass spectrometry
- gas chromatography
- minimally invasive
- antibiotic resistance genes