Application of liquid chromatography/electrospray ionization ion trap tandem mass spectrometry for the evaluation of global nucleic acids: methylation in garden cress under exposure to CuO nanoparticles.
Armando Alcazar MaganaKatarzyna WrobelAlma Rosa Corrales EscobosaKatarzyna WrobelPublished in: Rapid communications in mass spectrometry : RCM (2016)
The MRM quantification proposed here of cytosine-containing nucleosides using their proton-bound homo-dimers as precursor ions proved its utility for the assessment of global methylation of DNA and RNA in plants under stress imposed by CuO NPs. Detection of copper adducts with cytosine-containing ions, and their elimination by washing extracts with Cu(I) chelator, calls for further investigation.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- ultra high performance liquid chromatography
- high performance liquid chromatography
- mass spectrometry
- high resolution mass spectrometry
- gas chromatography
- simultaneous determination
- aqueous solution
- genome wide
- dna methylation
- quantum dots
- solid phase extraction
- high resolution
- circulating tumor
- water soluble
- oxide nanoparticles
- cell free
- single molecule
- loop mediated isothermal amplification
- electron transfer