Monoclonal Antibody-Based Colorimetric Lateral Flow Immunoassay for the Detection of Pyridaben in the Environment.
He ChenHao LiuYanran JiZekun ShaLi AnMeng LiDi ZhangXujin WuXiude HuaPublished in: Biosensors (2023)
Pyridaben, a broad-spectrum pyridazinone acaricide that is widely used in agricultural production, can induce neurotoxicity and reproductive abnormalities, and is highly toxic to aquatic organisms. In this study, a pyridaben hapten was synthesized and used to prepare monoclonal antibodies (mAbs), among which 6E3G8D7 showed the highest sensitivity in indirect competitive enzyme-linked immunosorbent assay, with a 50% inhibitory concentration (IC 50 ) of 3.49 ng mL -1 . The mAb, 6E3G8D7, was further applied to a gold nanoparticle-based colorimetric lateral flow immunoassay (CLFIA) for pyridaben detection, according to the signal intensity ratio of the test line to the control line, which showed a visual limit of detection of 5 ng mL -1 . The CLFIA also showed high specificity and achieved excellent accuracy in different matrices. In addition, the amounts of pyridaben in blind samples detected by the CLFIA, were consistent with high-performance liquid chromatography. Therefore, the developed CLFIA is considered a promising, reliable, and portable method for pyridaben on-site detection in agro-products and environmental samples.
Keyphrases
- label free
- loop mediated isothermal amplification
- monoclonal antibody
- sensitive detection
- high performance liquid chromatography
- risk assessment
- gold nanoparticles
- real time pcr
- nitric oxide
- high intensity
- human health
- high throughput
- heavy metals
- tandem mass spectrometry
- fluorescent probe
- single molecule
- multidrug resistant
- liquid chromatography
- low cost
- single cell