Development and comparison of RNA-sequencing pipelines for more accurate SNP identification: practical example of functional SNP detection associated with feed efficiency in Nellore beef cattle.

S LamJ ZeidanF MigliorA Suárez-VegaI Gómez-RedondoP A S FonsecaL L GuanS WatersAngela Cánovas

Published in: BMC genomics (2020)

The most optimized RNA-Seq pipeline allowed for more accurate identification of SNPs, associated positional candidate genes, and significantly associated metabolic pathways in muscle and liver tissues, providing insight on the underlying genetic architecture of feed efficiency in beef cattle.

Keyphrases

rna seq
single cell
genome wide
dna methylation
high resolution
bioinformatics analysis
copy number
gene expression
skeletal muscle
high density
label free
loop mediated isothermal amplification
genetic diversity