Assessing multiple conformations of lanthanide binding tags for proteins using a sensitive 19F-reporter.
Jia-Liang ChenBen-Guang ChenBin LiFeng YangXun-Cheng SuPublished in: Chemical communications (Cambridge, England) (2021)
Quantifying the isomeric species of metal complexes in solution is difficult. 19F NMR herein was used to determine the abundance of isomeric species and dynamic properties of lanthanide binding tags. The results suggest that 19F is an efficient reporter in assessing and screening paramagnetic tags suitable for protein NMR analysis.