Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.
Tristan C PaulKarl A JohnsonGuy M HagenPublished in: Bioengineering (Basel, Switzerland) (2023)
Super-resolution structured illumination microscopy (SR-SIM) is an optical fluorescence microscopy method which is suitable for imaging a wide variety of cells and tissues in biological and biomedical research. Typically, SIM methods use high spatial frequency illumination patterns generated by laser interference. This approach provides high resolution but is limited to thin samples such as cultured cells. Using a different strategy for processing raw data and coarser illumination patterns, we imaged through a 150-micrometer-thick coronal section of a mouse brain expressing GFP in a subset of neurons. The resolution reached 144 nm, an improvement of 1.7-fold beyond conventional widefield imaging.
Keyphrases
- high resolution
- high speed
- induced apoptosis
- single molecule
- mass spectrometry
- cell cycle arrest
- tandem mass spectrometry
- gene expression
- oxidative stress
- spinal cord
- endoplasmic reticulum stress
- spinal cord injury
- machine learning
- endothelial cells
- signaling pathway
- fluorescence imaging
- quantum dots
- pi k akt
- deep learning