Development of Aptamer-Based Molecular Tools for Rapid Intraoperative Diagnosis and In Vivo Imaging of Serous Ovarian Cancer.
Jing WangXiaona FangChenchen ZhangHaishuo JiQiushi PangXuqing LiZhaofeng LuoQiang WuLiyun ZhangPublished in: ACS applied materials & interfaces (2021)
Diagnosis and treatment of ovarian cancer are based on intraoperative pathology and debulking surgery. The development of a novel molecular tool is significant for rapid intraoperative pathologic diagnosis, which instructs the decision-making on excision surgery and effective chemotherapy. In this work, we represent a DNA aptamer named mApoc46, which is generated from cell-SELEX by targeting patient-derived primary serous ovarian cancer (pSOC) cells. An average dissociation constant (Kd) was determined to be 0.15 ± 0.05 μM by flow cytometry. The mApoc46 aptamer displays a robust specificity to pSOC cells. Labeled with FAM, mApoc46 can selectively stain living pSOC cells in 30 min without staining commercial OC cell lines and cell lines associated with other cancers. Interestingly, FAM-mApoc46 displayed superb selectivity toward high-grade serous ovarian cancer (HG-SOC) tissues in frozen sections against low-grade SOC, ovarian borderline tumor, other nonepithelial ovarian tumors, and healthy ovarian tissue. These results lead to a potential application in the identification of OCs' histological subtypes during operation. In the patient-derived tumor xenograft NCG mice model, Cy5-labeled mApoc46 was found to accumulate at the tumor area and served as an in vivo imaging probe. The mApoc46 probe shows a robust and stable performance to visualize SOC tumors in the body. Therefore, aptamer mApoc46 holds great potential in rapid intraoperative detection, pathological diagnosis, fluorescence image-guided cancer surgery, and targeted drug delivery and therapy.
Keyphrases
- high grade
- low grade
- induced apoptosis
- minimally invasive
- flow cytometry
- cell cycle arrest
- gold nanoparticles
- loop mediated isothermal amplification
- drug delivery
- sensitive detection
- coronary artery bypass
- label free
- patients undergoing
- endoplasmic reticulum stress
- decision making
- single molecule
- living cells
- neoadjuvant chemotherapy
- cell death
- squamous cell carcinoma
- signaling pathway
- magnetic nanoparticles
- oxidative stress
- locally advanced
- high resolution
- quantum dots
- mesenchymal stem cells
- gene expression
- cancer therapy
- acute coronary syndrome
- papillary thyroid
- rectal cancer
- pi k akt
- coronary artery disease
- pet imaging
- atrial fibrillation
- lymph node metastasis
- smoking cessation
- insulin resistance
- photodynamic therapy
- wild type
- aqueous solution
- fluorescence imaging