Rapid analysis of wheat gluten composition using a triple ELISA.
Clemens SchusterJulien HuenThomas WeissKatharina Anne ScherfPublished in: Journal of the science of food and agriculture (2024)
The quantitation of gliadins and HMW-GS by the experimental triple ELISA showed comparable results to RP-HPLC, whereas no strong correlation between the results from the two methods was found for LMW-GS. Overall, the experimental triple ELISA is suitable for relative gluten quantitation, especially for the analysis of large sample sets. Further work will focus on improving the experimental procedure of the ELISA. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Keyphrases
- ms ms
- mass spectrometry
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- monoclonal antibody
- tandem mass spectrometry
- solid phase extraction
- celiac disease
- simultaneous determination
- public health
- liquid chromatography
- climate change
- minimally invasive
- irritable bowel syndrome
- high resolution