In vitro PUVA treatment triggers calreticulin exposition and HMGB1 release by dying T lymphocytes in GVHD: New insights in extracorporeal photopheresis.

Céline CoppardDalil HannaniMarion HumbertVirginie GauthierJoel PlumasEtienne MerlinFrançoise GabertLaurence Chaperot

Published in: Journal of clinical apheresis (2019)

Altogether, in our experimental settings, in vitro PUVA-treatment induces a partially immunogenic phenotype allowing phagocytosis of apoptotic cells by macrophages and DC, however not sufficient to induce dendritic cell maturation and T cell activation. These data refine current models of ECP-mediated immune modulation and emphasize the need to further analyze PUVA-treated cell interactions with immune cells.

Keyphrases

dendritic cells
induced apoptosis
cell death
palliative care
single cell
cell therapy
regulatory t cells
cell cycle arrest
big data
acute lymphoblastic leukemia
combination therapy
oxidative stress
cell proliferation
endoplasmic reticulum stress
newly diagnosed