Quality Control of Next-Generation Sequencing-Based HIV-1 Drug Resistance Data in Clinical Laboratory Information Systems Framework.
Rupert CapinaKatherine LiLevon KearneyAnne-Mieke VandammeP Richard HarriganKristel Van LaethemPublished in: Viruses (2020)
Next-generation sequencing (NGS) in HIV drug resistance (HIVDR) testing has the potential to improve both clinical and public health settings, however it challenges the normal operations of quality management systems to be more flexible due to its complexity, massive data generation, and rapidly evolving protocols. While guidelines for quality management in NGS data have previously been outlined, little guidance has been implemented for NGS-based HIVDR testing. This document summarizes quality control procedures for NGS-based HIVDR testing laboratories using a laboratory information systems (LIS) framework. Here, we focus in particular on the quality control measures applied on the final sequencing product aligned with the recommendations from the World Health Organization HIV Drug Resistance Laboratory Network.
Keyphrases
- quality control
- antiretroviral therapy
- hiv positive
- hiv infected
- hiv testing
- human immunodeficiency virus
- public health
- hepatitis c virus
- electronic health record
- hiv aids
- men who have sex with men
- copy number
- south africa
- health information
- single cell
- quality improvement
- genome wide
- data analysis
- social media
- circulating tumor
- dna methylation
- artificial intelligence
- circulating tumor cells