Effect of Shear Stress on Pseudomonas aeruginosa Isolated from the Cystic Fibrosis Lung.
Jozef DingemansPieter MonsieursSung-Huan YuAurélie CrabbéKonrad U FörstnerAnne MalfrootPierre CornelisRob Van HoudtPublished in: mBio (2016)
Biofilm formation by Pseudomonas aeruginosa is one of the hallmarks of chronic cystic fibrosis (CF) lung infections. The biofilm matrix protects this bacterium from antibiotics as well as from the immune system. Hence, the prevention or reversion of biofilm formation is believed to have a great impact on treatment of chronic P. aeruginosa CF lung infections. In the present study, we showed that it is possible to modulate the behavior of a highly adapted transmissible P. aeruginosa CF isolate at both the transcriptomic and phenotypic levels by introducing shear stress in a CF-like environment, driving it from a biofilm to a planktonic lifestyle. Consequently, the results obtained in this study are of great importance with regard to therapeutic applications that introduce shear stress in the lungs of CF patients.
Keyphrases
- pseudomonas aeruginosa
- cystic fibrosis
- biofilm formation
- lung function
- acinetobacter baumannii
- staphylococcus aureus
- newly diagnosed
- end stage renal disease
- physical activity
- ejection fraction
- metabolic syndrome
- type diabetes
- prognostic factors
- single cell
- multidrug resistant
- drug resistant
- combination therapy
- drug induced