Nine jatrophane diterpenoids were isolated from the whole plant Euphorbia helioscopia , including two new ones, helioscopnins A ( 1 ) and B ( 2 ). Comprehensive spectroscopic data analysis and ECD calculations elucidated their structures, including absolute configurations. All compounds were evaluated for bioactivity towards autophagic flux by flow cytometry using HM mCherry-GFP-LC3 cells. Compounds 1 , 3 , 4 , 5 , 8 , and 9 significantly increased autophagic flux.
Keyphrases
- cell death
- data analysis
- flow cytometry
- cell cycle arrest
- induced apoptosis
- molecular docking
- density functional theory
- molecular dynamics simulations
- high resolution
- endoplasmic reticulum stress
- mass spectrometry
- simultaneous determination
- oxidative stress
- liquid chromatography
- cell proliferation
- pi k akt
- high resolution mass spectrometry