Cytotoxicity and alterations at transcriptional level caused by metals on fish erythrocytes in vitro.
Patricia MorcilloDiego RomeroJosé MeseguerM Ángeles EstebanAlberto CuestaPublished in: Environmental science and pollution research international (2016)
The in vitro use of fish erythrocytes to test the toxicity of aquatic pollutants could be a valuable alternative to fish bioassays but has received little attention. In this study, erythrocytes from marine gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens were exposed for 24 h to Cd, Hg, Pb and As and the resulting cytotoxicity was evaluated. Exposure to metals produced a dose-dependent reduction in the viability, and mercury showed the highest toxicity followed by MeHg, Cd, As and Pb. Moreover, fish erythrocytes incubated with each one of the metals exhibited alteration in gene expression profile of metallothionein, superoxide dismutase, catalase, peroxiredoxin, glutathione reductase, heat shock proteins 70 and 90, Bcl2-associated X protein and calpain1 indicating cellular protection, stress and apoptosis death as well as oxidative stress. This study points to the benefits for evaluating the toxicological mechanisms of marine pollution using fish erythrocytes in vitro.
Keyphrases
- oxidative stress
- heavy metals
- heat shock
- human health
- health risk assessment
- risk assessment
- health risk
- dna damage
- transcription factor
- heat stress
- working memory
- genome wide
- cell death
- gene expression
- particulate matter
- aqueous solution
- copy number
- induced apoptosis
- signaling pathway
- cell proliferation
- cell cycle arrest
- binding protein
- air pollution
- fine needle aspiration