Zika Virus Growth in Human Kidney Cells Is Restricted by an Elevated Glucose Level.
Alawiya ReslanJuliano G HaddadLiadrine Moukendza KoundiPhilippe DesprèsJean-Loup BascandsGilles GadeaPublished in: International journal of molecular sciences (2021)
Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain-Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival.
Keyphrases
- zika virus
- dengue virus
- high glucose
- endothelial cells
- aedes aegypti
- induced apoptosis
- cell cycle arrest
- human health
- sars cov
- blood glucose
- risk assessment
- pregnant women
- cell death
- escherichia coli
- oxidative stress
- signaling pathway
- blood pressure
- hiv infected
- combination therapy
- dna binding
- pi k akt
- case report
- transcription factor