Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA.

Mrityunjay GuptaJingtian WangChely M GarfioAbigail VandewalleRobert C Spitale

Published in: Chemical communications (Cambridge, England) (2023)

We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.

Keyphrases

transcription factor
single cell
nucleic acid
real time pcr
sensitive detection