Colletopeptides A-D, Anti-inflammatory Cyclic Tridepsipeptides from the Plant Endophytic Fungus Colletotrichum sp. S8.
Li FengJia WangShuai LiuXue-Jia ZhangQi-Rui BiYan-Yun HuZhe WangNing-Hua TanPublished in: Journal of natural products (2019)
Four new hybrid peptide-polyketide cyclic tridepsipeptides, colletopeptides A-D (1-4), were isolated and characterized from the endophytic fungus Colletotrichum sp. S8 derived from the stems of Rubia podantha with the guidance of LC-UV-MS detection. Their structures were elucidated by extensive spectroscopic analysis and X-ray crystallography. Compounds 1-4 are rare natural 12-membered cyclic tridepsipeptides containing a 3,5,11-trihydroxy-2-methyl dodecanoic acid unit in their structures. 1-4 inhibited lipopolysaccharide-induced nitric oxide production in RAW264.7 macrophages with the IC50 values of 8.3, 38.7, 13.5, and 22.2 μM, respectively. 1 also inhibited the production of inflammatory factors IL-6 and TNF-α, and decreased the phosphorylation of NF-κB-associated proteins IκBα and p65.
Keyphrases
- lipopolysaccharide induced
- nitric oxide
- high resolution
- inflammatory response
- anti inflammatory
- mass spectrometry
- oxidative stress
- lps induced
- signaling pathway
- rheumatoid arthritis
- multiple sclerosis
- molecular docking
- ms ms
- nitric oxide synthase
- hydrogen peroxide
- simultaneous determination
- pi k akt
- nuclear factor
- computed tomography
- immune response
- dual energy
- protein kinase
- liquid chromatography
- electron microscopy