MoS 2 -Carbon Nanodots as a New Electrochemiluminescence Platform for Breast Cancer Biomarker Detection.
Laura Gutiérrez-GálvezManuel Vázquez SulleiroCristina Gutiérrez-SánchezDaniel García-NietoMonica LunaEmilio M PérezTania García-MendiolaMaría Encarnación LorenzoPublished in: Biosensors (2023)
In this work, we present the combination of two different types of nanomaterials, 2D molybdenum disulfide nanosheets (MoS 2 -NS) and zero-dimensional carbon nanodots (CDs), for the development of a new electrochemiluminescence (ECL) platform for the early detection and quantification of the biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS 2 -NS are used as an immobilization platform for the thiolated aptamer, which can recognize the HER2 epitope peptide with high affinity, and CDs act as coreactants of the anodic oxidation of the luminophore [Ru(bpy) 3 ] 2+ . The HER2 biomarker is detected by changes in the ECL signal of the [Ru(bpy) 3 ] 2+ /CD system, with a low detection limit of 1.84 fg/mL and a wide linear range. The proposed method has been successfully applied to detect the HER2 biomarker in human serum samples.
Keyphrases
- quantum dots
- sensitive detection
- energy transfer
- epidermal growth factor receptor
- visible light
- loop mediated isothermal amplification
- high throughput
- reduced graphene oxide
- room temperature
- label free
- endothelial cells
- gold nanoparticles
- dengue virus
- cell proliferation
- highly efficient
- transition metal
- nitric oxide
- hydrogen peroxide
- zika virus
- induced pluripotent stem cells
- real time pcr
- breast cancer risk