A comparative study of next-generation sequencing and fragment analysis for the detection and allelic ratio determination of FLT3 internal tandem duplication.
Jin Ju KimKwang Seob LeeTaek Gyu LeeSeungjae LeeSaeam ShinSeung-Tae LeePublished in: Diagnostic pathology (2022)
Sensitive FLT3 ITD detection with comprehensive information of other mutation offered by NGS could be a useful tool in clinical laboratories. Future studies will be needed to evaluate and standardize NGS AF cutoff to predict actual clinical outcomes.