Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818).
Hoda Al AhmedBernadeth F TicarIan BlackFakhri MahdiAnter A ShamiSandeep K MisraChristian HeissJason J ParisJoshua S SharpParastoo AzadiVitor H PominPublished in: Glycoconjugate journal (2022)
Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D 1 H, 1 H- 1 H COSY, 1 H- 1 H TOCSY, 1 H- 1 H NOESY, 1 H- 13 C HSQC and 1 H- 13 C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina-derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H 2 O 2 - and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H 2 O 2 - and LPS-induced ROS, and potent cell proliferative activity.
Keyphrases
- lps induced
- inflammatory response
- magnetic resonance
- reactive oxygen species
- induced apoptosis
- gas chromatography mass spectrometry
- cell cycle arrest
- high resolution
- cell proliferation
- cell death
- mass spectrometry
- single cell
- high speed
- climate change
- anti inflammatory
- dna damage
- oxidative stress
- cell therapy
- tandem mass spectrometry
- toll like receptor
- high performance liquid chromatography
- high throughput
- type diabetes
- genome wide
- adipose tissue
- signaling pathway
- gene expression
- cell cycle
- metabolic syndrome
- endoplasmic reticulum stress
- computed tomography
- risk assessment
- heat shock protein
- dna methylation
- contrast enhanced
- blood pressure
- stem cells
- gas chromatography
- human health
- ms ms
- cell surface