Corymbulosins D-H, 2-Hydroxy- and 2-Oxo-clerodane Diterpenes from the Bark of Laetia corymbulosa.
Airi SuzukiYohei SaitoShuichi FukuyoshiMasuo GotoKatsunori MiyakeDavid J NewmanBarry R O'KeefeKuo-Hsiung LeeKyoko Nakagawa GotoPublished in: Journal of natural products (2017)
A bioactive CH3OH-CH2Cl2 (1:1) extract of the bark of Laetia corymbulosa provided five new clerodane diterpenes with an isozuelanin skeleton, designated as corymbulosins D-H (1-5), as well as the known corymbulosins B (6) and C (7), for which the relative configurations were not previously determined. The structures of 1-5 were characterized on the basis of 1D and 2D NMR spectroscopic and HRMS analysis. The absolute configurations of all isolated compounds 1-7 were verified through chemical methods, including modified Mosher esterifications or oxidation of the hydroxy group at C-2, ECD experiments, and spectroscopic data comparison. The isolated compounds were evaluated for antiproliferative activity against a small panel of human cancer cell lines.
Keyphrases
- molecular docking
- high resolution
- endothelial cells
- room temperature
- papillary thyroid
- magnetic resonance
- oxidative stress
- electronic health record
- big data
- hydrogen peroxide
- squamous cell carcinoma
- anti inflammatory
- young adults
- mass spectrometry
- molecular dynamics simulations
- childhood cancer
- high resolution mass spectrometry
- lymph node metastasis
- artificial intelligence
- simultaneous determination
- ionic liquid
- liquid chromatography