Scanning Switch-off Microscopy for Super-Resolution Fluorescence Imaging.
Zhaoshuai GaoShangguo HouSuhui DengLe LiangFei WangLinjie GuoWeina FangQian LiBin KangHong-Yuan ChenChun-Hai FanPublished in: Nano letters (2024)
Super-resolution (SR) microscopy provides a revolutionary optical imaging approach by breaking the diffraction limit of light, while the commonly required special instrumentation with complex optical setup hampers its popularity. Here, we present a scanning switch-off microscopy (SSM) concept that exploits the omnipresent switch-off response of fluorophores to enable super-resolution imaging using a commercial confocal microscope. We validated the SSM model with theoretical calculations and experiments. An imaging resolution of ∼100 nm was obtained for DNA origami nanostructures and cellular cytoskeletons using fluorescent labels of Alexa 405, Alexa 488, Cy3, and Atto 488. Notably, super-resolution imaging of live cells was realized with SSM, by employing a dronpa fluorescent protein as the fluorescent label. In principle, this SSM method can be applied to any excitation laser scanning-based microscope.
Keyphrases
- high resolution
- fluorescence imaging
- high speed
- single molecule
- mass spectrometry
- quantum dots
- label free
- photodynamic therapy
- living cells
- optical coherence tomography
- high throughput
- induced apoptosis
- molecular dynamics simulations
- oxidative stress
- cell proliferation
- amino acid
- raman spectroscopy
- density functional theory
- fluorescent probe