Ameliorative effect of selenium nanoparticles on the structure and function of testis and in vitro embryo development in Aflatoxin B1-exposed male mice.

The purpose of the research was to investigate the therapeutic ability of selenium nanoparticles (Se-NPs) on the aflatoxin B1 (AFB1) toxicity induced in the male reproductive system. For this experiment, the mature male mice were put into four groups. Control (0.5 ml PBS, 60 days; IP, n = 7), Se-NPs (0.5 µg kg-1  day-1 for 60 days; IP), AFB1 (4.5 mg kg-1  day-1 for 60 days; IP) and AFB1 + Se-NPs (4.5 mg kg-1  day-1  + 0.5 µg kg-1  day-1 for 60 days; IP). After treatment, the histological structure of testis, serum testosterone level and sperm parameters, including concentration, motility, viability, morphology and DNA fragmentation, were examined. The results demonstrated that the AFB1 destroyed the testicular tissue structure and decreased the sperm concentration, motility, viability and normal morphology significantly. AFB1 also could significantly increase sperm DNA fragmentation and reduce in vitro fertilisation and embryo development compared to the control group (p < .001). Our data show that Se-NPs could inhibit AFB1-induced damage to the testis and improve sperm parameters as well as in vitro fertilisation and embryo production in AFB1 exposed male mice. This study revealed that the administration of Se-NPs could attenuate the testicular injury of AFB1 and improve the male reproductive system function in AFB1 exposed mice.