IGF-1 Peptide Mimetic-functionalized Hydrogels Enhance MSC Survival and Immunomodulatory Activity.
Xiaohong TanLiufang JingSydney M NealMunish C GuptaJacob M BuchowskiLori A SettonNathaniel HuebschPublished in: bioRxiv : the preprint server for biology (2024)
Insulin-like growth factor 1 (IGF-1) plays a multifaceted role in stem cell biology and may promote proliferation, survival, migration, and immunomodulation for MSCs. In this study, we functionalized alginate hydrogels with integrin-binding and IGF-1 peptide mimetics to investigate their impact on MSC function. Embedding MSCs in these hydrogels enhanced their ability to reduce inflammatory cytokine production and promote anti-inflammatory gene expression in cells from degenerative human intervertebral discs exposed to proteins secreted by the MSC. This approach suggests a new way to retain and augment MSC functionality using IGF-1 peptide mimetics, offering an alternative to co-delivery of cells and high dose soluble growth factors for tissue repair and immune- system modulation.
Keyphrases
- growth hormone
- binding protein
- gene expression
- pi k akt
- high dose
- drug delivery
- stem cells
- tissue engineering
- hyaluronic acid
- wound healing
- mesenchymal stem cells
- cell cycle arrest
- signaling pathway
- induced apoptosis
- drug release
- anti inflammatory
- extracellular matrix
- endothelial cells
- quantum dots
- umbilical cord
- low dose
- oxidative stress
- stem cell transplantation
- molecularly imprinted
- endoplasmic reticulum stress
- transcription factor
- cell therapy
- high resolution