Prolonged Scar-in-a-Jar: an in vitro screening tool for anti-fibrotic therapies using biomarkers of extracellular matrix synthesis.
Sarah Rank RønnowRand Qais DabbaghFederica GenoveseCarmel B NanthakumarVikki J BarrettRobert B GoodSarah BrockbankSimon CruwysHenrik JessenGrith Lykke SorensenMorten Asser KarsdalDiana Julie LeemingJannie Marie Bülow SandPublished in: Respiratory research (2020)
TGF-β1 stimulated synthesis of type I, III and VI collagen, fibronectin and α-SMA but not type IV or V collagen. Synthesis was increased over time, although temporal profiles differed, and was modulated pharmacologically by ALK5i, nintedanib, pirfenidone and omipalisib. This prolonged 12-day Scar-in-a-Jar assay utilising biochemical markers of ECM synthesis provides a useful screening tool for novel anti-fibrotic compounds.