Assay for improved detection of antigen-specific immune cells from extrasanguinous fluids.

David SchubMathias FousseJulia ElsäßerKlaus FaßbenderUrban SesterTina SchmidtMartina Sester

Published in: European journal of immunology (2018)

In this approach, pre-stained cells from extrasanguinous fluids (ESFs) are stimulated in the presence of blood from the same individual. Thus, blood-derived antigen-presenting cells enable stimulation of both ESF- and blood T cells. Pre-staining allows distinction of T cells from ESF and blood, and simultaneous analysis of antigen-specific T cells in both compartments.

Keyphrases

induced apoptosis
high throughput
cell death
signaling pathway
real time pcr
label free
flow cytometry
sensitive detection
pi k akt