Turn-On Assay for HIV-1 Protease Inhibitor Selection.
Yan ShiZhuang LiHongda WangPublished in: ACS applied bio materials (2020)
Virus threatens our lives and health at all times. Among them, HIV has caused millions of deaths since its discovery. Research on viral inhibitor selection has become an urgent need. A reliable turn-on assay for HIV-1 protease (HIV-1 PR) inhibitor selection has been developed. The feasibility assay of protease detection was demonstrated based on fluorescence resonance energy transfer between graphene oxide and dye-modified peptides. Besides the rapid and sensitive detection of HIV-1 PR, the enzyme kinetic parameters were also measured. Most importantly, this assay was applied in the distinction of protease inhibitor efficiency and the unknown inhibitor selection.
Keyphrases
- antiretroviral therapy
- hiv positive
- hiv infected
- hiv testing
- sensitive detection
- human immunodeficiency virus
- energy transfer
- hepatitis c virus
- hiv aids
- high throughput
- men who have sex with men
- loop mediated isothermal amplification
- quantum dots
- south africa
- healthcare
- mental health
- sars cov
- small molecule
- living cells
- risk assessment
- single cell
- label free
- human health
- climate change