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Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis.

Shan ChenTao JiangHao LinJunyan ChenShuangli YangPengcheng WangXinqiang GanYali WangBin XuJunjie SunCongcong YinZongxiong HuangYi-Min Fang
Published in: Analytical chemistry (2021)
Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 × 103 particles/μL in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost.
Keyphrases
  • mesenchymal stem cells
  • prostate cancer
  • stem cells
  • low cost
  • loop mediated isothermal amplification
  • real time pcr
  • gold nanoparticles
  • mass spectrometry
  • sensitive detection
  • high resolution
  • structural basis