High-Throughput Metabolomics using 96-plex Isotope Tagging.
Michael R ArmbrusterScott F GradyKevin ChoGary J PattiBenjamin J BythellChristopher K ArnattJames L EdwardsPublished in: Analytical chemistry (2024)
Liquid chromatography-mass spectrometry (LC-MS) based metabolomics suffers from extended duty cycles and matrix-dependent quantitation. Chemical tags with 96 unique masses are reported, which alleviate the metabolomic workflow bottleneck and allow for absolute quantitation. A metabolic screen for carboxylic acids was performed on mammalian cells deprived of various nutrients and showed 24% RSD and analysis of 288 samples in 2 h.
Keyphrases
- mass spectrometry
- liquid chromatography
- high throughput
- gas chromatography
- tandem mass spectrometry
- high resolution mass spectrometry
- high performance liquid chromatography
- capillary electrophoresis
- high resolution
- single cell
- simultaneous determination
- solid phase extraction
- heavy metals
- electronic health record
- fine needle aspiration
- liquid chromatography tandem mass spectrometry