The use of commercial fibrin glue in dermal replacement material reduces angiogenic and lymphangiogenic gene and protein expression in vitro.
Benedikt FuchsAlexandra BirtNicholas MoellhoffConstanze KuhlmannRiccardo GiuntaPaul Severin WiggenhauserPublished in: Journal of biomaterials applications (2023)
Our findings demonstrate the impact of fibrin glue application in dermal regeneration with special regard to angiogenesis and lymphangiogenesis. In particular, a short fibrin treatment of 24 hours led to a decrease in gene and protein levels of LECS, HUVECs, and ASCs. In contrast, the long-term application showed less effect on gene and protein expressions. Therefore, this work demonstrated the negative effects of fibrin-treated cells in tissue engineering approaches and could affect wound healing during dermal regeneration.
Keyphrases
- wound healing
- tissue engineering
- stem cells
- genome wide
- copy number
- induced apoptosis
- genome wide identification
- platelet rich plasma
- magnetic resonance
- binding protein
- magnetic resonance imaging
- endothelial cells
- small molecule
- protein protein
- computed tomography
- gene expression
- endoplasmic reticulum stress
- cell proliferation
- transcription factor
- genome wide analysis
- smoking cessation
- replacement therapy