Electrochemiluminescence Microscopy of Cells: Essential Role of Surface Regeneration.
Dongni HanBertrand GoudeauDe-Chen JiangDanjun FangNeso SojicPublished in: Analytical chemistry (2020)
Electrochemiluminescence (ECL) microscopy is successfully applied to image cells, micro-/nano-objects, and electrochemical processes at electrode surfaces. The classic ECL tandem system is composed of the [Ru(bpy)3]2+ luminophore with the very efficient tripropylamine (TPA) coreactant. The dramatic decrease of the ECL signal observed when recording successive ECL images constitutes a key limitation for the development of ECL microscopy. Herein, we investigated the progressive decrease of the ECL signal of Chinese hamster ovary (CHO) cells. The plasma membranes of CHO cells were labeled with a [Ru(bpy)3]2+ derivative, and the ECL images were recorded using the TPA coreactant. We demonstrate that the loss of the ECL signal is related to the electrochemical step because of a progressive lower TPA oxidation current. We tested a cathodic regenerative treatment of the electrode surface, which allowed us to restore the initial TPA oxidation intensity and thus to record a sequence of ECL images without any vanishing of the light signal. The electrochemical approach presented here is an essential step for the development of ECL microscopy of cells.
Keyphrases
- induced apoptosis
- cell cycle arrest
- optical coherence tomography
- deep learning
- high resolution
- stem cells
- single molecule
- endoplasmic reticulum stress
- mesenchymal stem cells
- computed tomography
- staphylococcus aureus
- cell death
- pseudomonas aeruginosa
- hydrogen peroxide
- cell proliferation
- machine learning
- convolutional neural network
- mass spectrometry
- quantum dots
- pet ct
- smoking cessation
- liquid chromatography
- tissue engineering