Mechanosensitivity is an essential component of phototransduction in vertebrate rods.
Ulisse BoccheroFabio FalleroniSimone MortalYunzhen LiDan CojocTrevor LambVincent TorrePublished in: PLoS biology (2020)
Photoreceptors are specialized cells devoted to the transduction of the incoming visual signals. Rods are able also to shed from their tip old disks and to synthesize at the base of the outer segment (OS) new disks. By combining electrophysiology, optical tweezers (OTs), and biochemistry, we investigate mechanosensitivity in the rods of Xenopus laevis, and we show that 1) mechanosensitive channels (MSCs), transient receptor potential canonical 1 (TRPC1), and Piezo1 are present in rod inner segments (ISs); 2) mechanical stimulation-of the order of 10 pN-applied briefly to either the OS or IS evokes calcium transients; 3) inhibition of MSCs decreases the duration of photoresponses to bright flashes; 4) bright flashes of light induce a rapid shortening of the OS; and 5) the genes encoding the TRPC family have an ancient association with the genes encoding families of protein involved in phototransduction. These results suggest that MSCs play an integral role in rods' phototransduction.
Keyphrases
- mesenchymal stem cells
- umbilical cord
- genome wide
- induced apoptosis
- vascular smooth muscle cells
- cell cycle arrest
- high resolution
- genome wide identification
- bioinformatics analysis
- binding protein
- bone marrow
- cerebral ischemia
- risk assessment
- brain injury
- small molecule
- blood brain barrier
- high speed
- cell free
- climate change
- human health
- pi k akt
- protein protein
- subarachnoid hemorrhage
- loop mediated isothermal amplification
- light emitting