Molecular Identification of Sarcocystis Species in Sheep from Lithuania.

Data on the distribution of different Sarcocystis species in various muscles of sheep are scarce. In the present study, 190 diaphragm, oesophagus, and heart muscle samples of 69 sheep raised in Lithuania were examined for the presence of Sarcocystis spp. Under a light microscope, two morphological types of microcysts corresponding to S . arieticanis and S . tenella were detected. Eight and 12 sarcocysts of S . arieticanis and S . tenella , respectively, were isolated and characterised by the sequencing of a portion of cox1 . The sequence comparisons revealed the highest similarity between European and Asian isolates of S . arieticanis and S . tenella obtained from domestic sheep and other wild Caprinae hosts. Based on peptic digestion, nested PCR targeting cox1, and sequencing, a 100% infection prevalence of S . arieticanis and S . tenella was observed in the 69 studied animals. The occurrence of S . tenella was significantly higher in the diaphragm than in the oesophagus (χ2 = 13.14, p < 0.001), whereas differences in the prevalence of S . arieticanis in the studied muscle types were insignificant (χ2 = 1.28, p > 0.05). Further molecularly based epidemiological studies are needed to compare the prevalence of Sarcocystis species in various muscles of sheep raised in different geographic regions.