PNA-Based MicroRNA Detection Methodologies.
Enrico CadoniAlex ManicardiAnnemieke MadderPublished in: Molecules (Basel, Switzerland) (2020)
MicroRNAs (miRNAs or miRs) are small noncoding RNAs involved in the fine regulation of post-transcriptional processes in the cell. The physiological levels of these short (20-22-mer) oligonucleotides are important for the homeostasis of the organism, and therefore dysregulation can lead to the onset of cancer and other pathologies. Their importance as biomarkers is constantly growing and, in this context, detection methods based on the hybridization to peptide nucleic acids (PNAs) are gaining their place in the spotlight. After a brief overview of their biogenesis, this review will discuss the significance of targeting miR, providing a wide range of PNA-based approaches to detect them at biologically significant concentrations, based on electrochemical, fluorescence and colorimetric assays.
Keyphrases
- label free
- nucleic acid
- gold nanoparticles
- loop mediated isothermal amplification
- real time pcr
- cell proliferation
- single molecule
- long non coding rna
- papillary thyroid
- single cell
- gene expression
- air pollution
- transcription factor
- cell therapy
- fluorescent probe
- squamous cell
- cancer therapy
- sensitive detection
- squamous cell carcinoma
- stem cells
- ionic liquid
- nitric oxide
- living cells
- drug delivery
- heat shock
- mesenchymal stem cells
- electron transfer