New Indole Diketopiperazine Alkaloids from Soft Coral-Associated Epiphytic Fungus Aspergillus sp. EGF 15-0-3.
Xia WeiChan FengSi-Yu WangDong-Mei ZhangXiao-Hui LiCui-Xian ZhangPublished in: Chemistry & biodiversity (2020)
Three new indole diketopiperazine alkaloids, 11-methylneoechinulin E and variecolorin M, and (+)-variecolorin G, along with 12 known analogs, were isolated from a soft coral-associated epiphytic fungus Aspergillus sp. EGF 15-0-3. The structures of the new compounds were unambiguously established by extensive spectroscopic analyses including HR-ESI-MS, 1D and 2D NMR spectroscopy and optical rotation measurements. The absolute configurations of (+)- and (-)-variecolorin G were determined by experimental and quantum-chemical ECD investigations and single-crystal X-ray diffraction analysis. Variecolorin G is a pair of enantiomeric mixtures with a ratio of 1 : 2. Moreover, (+)-neoechinulin A is firstly reported as a natural product. The cytotoxic activities of all the isolated compounds against NCI-H1975 gefitinib resistance (NCI-H1975/GR) cell lines were preliminarily evaluated by MTT method.
Keyphrases
- high resolution
- ms ms
- molecular docking
- growth factor
- mass spectrometry
- small cell lung cancer
- multiple sclerosis
- molecular dynamics
- cell wall
- electron microscopy
- epidermal growth factor receptor
- magnetic resonance
- capillary electrophoresis
- computed tomography
- energy transfer
- tyrosine kinase
- anti inflammatory
- data analysis