Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy.

Michael TrumppAnna OliverasHannes GonschiorJulia Ast David J Hodson Petra KnausMartin LehmannMelissa BirolJohannes Broichhagen

Published in: Chemical communications (Cambridge, England) (2022)

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.

Keyphrases

single molecule
energy transfer
cell surface
quantum dots
induced apoptosis
living cells
atomic force microscopy
cell cycle arrest
protein protein
endoplasmic reticulum stress
convolutional neural network
binding protein
small molecule
high throughput
deep learning
single cell
pi k akt
neural network
solid state