A genetic variant in microRNA-146a is associated with sporadic breast cancer in a Southern Brazilian Population.
Heloisa Magagnin BrincasDanillo G AugustoCarolina MathiasIglenir João CavalliRubens Silveira de LimaFlávia KurodaCícero de Andrade UrbanDaniela Fiori GradiaJaqueline de OliveiraRodrigo Coutinho de AlmeidaEnilze Maria de Souza Fonseca RibeiroPublished in: Genetics and molecular biology (2020)
MicroRNAs (miRNAs) play an essential role in gene expression and affect the development of tumours, including breast cancer (BC). Polymorphisms in miRNA genes can affect the interaction of miRNAs with their target messenger RNA by interfering, creating or disrupting target sites. The single nucleotide polymorphism (SNP) rs2910164, located in the seed region of miR146a, was shown to be associated with BC among different populations. In the present study, we investigated whether rs2910164 is associated with BC in 326 patients and 411 controls from a Brazilian population of predominantly European ancestry. The presence of the allele rs2910164*C was associated with an increased risk of BC (OR=1.4, 95% CI=1.03-1.85, p = 0.03). We also analysed publicly available RNA-seq data to evaluate if miR146a is differentially expressed in different subtypes of BC. Genotyping was performed by polymerase chain reaction with sequence-specific primers (PCR-SSP). By leveraging public data from TCGA database, we analysed 461 patients and found that miR146a is significantly more expressed in BC than in non-tumor tissue (1.47 fold, p = 0.02) and is expressed to a greater degree in aggressive BC subtypes.
Keyphrases
- end stage renal disease
- gene expression
- rna seq
- cell proliferation
- long non coding rna
- genome wide
- ejection fraction
- newly diagnosed
- chronic kidney disease
- single cell
- prognostic factors
- dna methylation
- long noncoding rna
- healthcare
- peritoneal dialysis
- young adults
- deep learning
- copy number
- early onset
- single molecule