Biocatalytic synthesis of 2-O-α-D-glucopyranosyl-L-ascorbic acid using an extracellular expressed α-glucosidase from Oryza sativa.

The production of rAGL in P. pastoris was proved to be beneficial in providing enough enzyme and promoting biocatalytic synthesis of AA-2G. These studies lay the basis for the industrial application of α-glucosidase.