A mechanism for epigenetic control of DNA replication.

Courtney G SansamKatarzyna PietrzakBlanka MajchrzyckaMaciej A Kerlin Jingrong Chen Susannah Rankin Christopher L Sansam

Published in: Genes & development (2018)

DNA replication origins in hyperacetylated euchromatin fire preferentially during early S phase. However, how acetylation controls DNA replication timing is unknown. TICRR/TRESLIN is an essential protein required for the initiation of DNA replication. Here, we report that TICRR physically interacts with the acetyl-histone binding bromodomain (BRD) and extraterminal (BET) proteins BRD2 and BRD4. Abrogation of this interaction impairs TICRR binding to acetylated chromatin and disrupts normal S-phase progression. Our data reveal a novel function for BET proteins and establish the TICRR-BET interaction as a potential mechanism for epigenetic control of DNA replication.

Keyphrases

dna methylation
genome wide
gene expression
binding protein
big data
risk assessment
small molecule
protein protein
amino acid
dna binding
machine learning
histone deacetylase