Optimisation of the preservation conditions for molecularly imprinted polymer nanoparticles specific for trypsin.
Abeer H M SafaryanAdam M SmithThomas S BedwellElena V PiletskaFrancesco CanfarottaSergey A PiletskyPublished in: Nanoscale advances (2019)
The influence of lyophilisation, autoclaving and sonication on the stability and performance of trypsin-specific molecularly imprinted polymer nanoparticles (MIP NPs) has been studied in order to improve their long-term physical stability. Glucose, glycine, sorbitol and trehalose were tested as cryoprotectant agents during the lyophilisation treatment. The effect of lyophilisation and sterilisation on affinity of trypsin-specific NPs was assessed using Biacore 3000 instrument. The results have demonstrated that MIP NPs successfully withstood the lyophilisation and autoclaving conditions without a reduction of their recognition properties and affinity. It is possible to conclude that both tested lyophilisation and sterilisation treatments were suitable for a long-term storage of the prepared MIP NPs and could be used to store MIP NPs in dry state and hence reduce the chance of the bacterial contamination. An effective preservation of the MIP NPs is a crucial requirement for their future applications in the clinical diagnostics and bioimaging.
Keyphrases
- molecularly imprinted
- oxide nanoparticles
- solid phase extraction
- risk assessment
- mental health
- type diabetes
- physical activity
- health risk
- drinking water
- metabolic syndrome
- blood pressure
- current status
- heavy metals
- fluorescent probe
- living cells
- mass spectrometry
- insulin resistance
- capillary electrophoresis
- climate change
- walled carbon nanotubes
- tandem mass spectrometry
- combination therapy