DNA-Templated Silver Nanocluster/Porphyrin/MnO2 Platform for Label-Free Intracellular Zn2+ Imaging and Fluorescence-/Magnetic Resonance Imaging-Guided Photodynamic Therapy.
Yao YaoNa LiXing ZhangJeremiah Ong'achwa MachukiDongzhi YangYanyan YuJingjing LiDaoquan TangJiangwei TianFenglei GaoPublished in: ACS applied materials & interfaces (2019)
Developing a theranostic platform that integrates diagnosis and treatment in one single nanostructure is necessary for efficient tumor treatment. Here, we presented a novel theranostic nanoprobe for nonlabeled fluorescence imaging of Zn2+ and 635 nm red light-triggered photodynamic therapy (PDT) by a multifunctional DNA-templated silver nanocluster/porphyrin/MnO2 nanoplatform. MnO2 nanosheets adsorbed hairpin DNA-silver nanoclusters (AgNCs) and porphyrin (P) by facile physisorption, which accelerate the transfection of nanoprobes and P into tumor cells. After entering the cells, the biodegradation of MnO2 nanosheets by glutathione and acidic hydrogen peroxide released AgNCs for label-free Zn2+ fluorescence imaging by the hairpin DNA-fueled dynamic self-assembly of three-way DNA junction architectures, and the released Mn2+ could act as an effective magnetic resonance imaging (MRI) contrast agent. In addition, MnO2 was decomposed in the acidic H2O2-ample environment and produced O2 to overbear hypoxia-related PDT resistance, highly efficient PDT was obtained by excess singlet oxygen (1O2) release of P-AgNCs-MnO2 nanoprobes under light irradiation compared with free P. In vitro and in vivo studies confirmed that P-AgNCs-MnO2 exhibited high fluorescence specificity, excellent PDT effect, and good biocompatibility and could be used as a contrast agent for MRI. This theranostic platform provided a new avenue for the fluorescence and MRI diagnosis of tumors and efficient tumor treatment.
Keyphrases
- photodynamic therapy
- fluorescence imaging
- magnetic resonance imaging
- single molecule
- label free
- contrast enhanced
- highly efficient
- circulating tumor
- cell free
- hydrogen peroxide
- gold nanoparticles
- energy transfer
- diffusion weighted imaging
- magnetic resonance
- computed tomography
- high throughput
- heavy metals
- nucleic acid
- living cells
- induced apoptosis
- reduced graphene oxide
- quantum dots
- nitric oxide
- cell cycle arrest
- cell proliferation
- sensitive detection
- single cell
- mass spectrometry
- reactive oxygen species