Bacteria eat nanoprobes for aggregation-enhanced imaging and killing diverse microorganisms.
Yunmin YangBinbin ChuJiayi ChengJiali TangBin SongHouyu WangYao HePublished in: Nature communications (2022)
Currently optical-based techniques for in vivo microbial population imaging are limited by low imaging depth and highly light-scattering tissue; and moreover, are generally effective against only one specific group of bacteria. Here, we introduce an imaging and therapy strategy, in which different bacteria actively eat the glucose polymer (GP)-modified gold nanoparticles through ATP-binding cassette (ABC) transporter pathway, followed by laser irradiation-mediated aggregation in the bacterial cells. As a result, the aggregates display ~15.2-fold enhancement in photoacoustic signals and ~3.0-fold enhancement in antibacterial rate compared with non-aggregated counterparts. Significantly, the developed strategy allows ultrasensitive imaging of bacteria in vivo as low ~10 5 colony-forming unit (CFU), which is around two orders of magnitude lower than most optical contrast agents. We further demonstrate the developed strategy enables the detection of ~10 7 CFU bacteria residing within tumour or gut. This technique enables visualization and treatment of diverse bacteria, setting the crucial step forward the study of microbial ecosystem.
Keyphrases
- high resolution
- gold nanoparticles
- fluorescence imaging
- microbial community
- type diabetes
- induced apoptosis
- climate change
- radiation therapy
- magnetic resonance
- blood pressure
- photodynamic therapy
- mass spectrometry
- magnetic resonance imaging
- metabolic syndrome
- quantum dots
- bone marrow
- weight loss
- endoplasmic reticulum stress
- wound healing
- glycemic control
- blood glucose
- smoking cessation