Isolation, Cytotoxicity, and In-silico Screening of Coumarins from Psoralea corylifolia Linn.
Nancy TripathiAarati ParmarNilesh PandeyNivedita BhardwajSanheeta ChakrabartyRuma SarkarHemant KumarShreyans K JainPublished in: Chemistry & biodiversity (2024)
Psoralea corylifolia (syn. Cullen corylifolium), commonly called bawachi, is a medicinal plant extensively used for skin conditions like leukoderma, vitiligo, and psoriasis. It is notably rich in valuable bioactive compounds, particularly coumarins and furanocoumarins. This study isolated fourteen coumarins from P. corylifolia which were tested for cytotoxicity using the MTT assay, with compound 10 showing good cytotoxicity against A549 cells (IC 50 0.9 μM), while compound 1, compound 2, and compound 3 displaying potential cytotoxicity against MDA-MB-231 cells (IC 50 0.49 μM, 0.56 μM, and 0.84 μM respectively). Additionally, the compounds' interaction with Epidermal Growth Factor Receptor (EGFR) protein, highly expressed in both cell lines, was investigated through molecular modeling studies, that aligned well with cytotoxicity results. The findings revealed the remarkable cytotoxic potential of four coumarins 1, 2, 3, and 10 against A549 and MDA-MB-231 cell lines.
Keyphrases
- epidermal growth factor receptor
- cell cycle arrest
- induced apoptosis
- tyrosine kinase
- small cell lung cancer
- advanced non small cell lung cancer
- cell death
- breast cancer cells
- pi k akt
- multidrug resistant
- high throughput
- single cell
- oxidative stress
- molecular docking
- protein protein
- amino acid
- soft tissue
- atomic force microscopy
- drug discovery