Comparison between next-generation sequencing and multiplex polymerase chain reaction assays for nonsmall-cell lung cancer molecular diagnosis.

Shuji MurakamiKanako ShinadaYuka OtsutsumiFumiko KomineYuan YuanJunko NakamuraSeigo KatakuraTetsuro KondoTerufumi KatoTomoyuki YokoseHaruhiro Saito

Published in: Cancer medicine (2024)

The AmoyDx PLC panel, a PCR-based multiplex diagnostic test, exhibits a high success rate. The frequency of the nine genes targeted for treatment detected by the AmoyDx PLC panel was comparable to the frequency of mutations detected by ODxTT-M. Clinicians should understand and use the AmoyDx PLC panel and ODxTT-M with respect to their respective performances and limitations.

Keyphrases

high throughput
real time pcr
single cell
genome wide
palliative care
epidermal growth factor receptor
cell therapy
copy number
stem cells
gene expression
bone marrow
dna methylation
circulating tumor
cell free