Development of RNA-FISH Assay for Detection of Oncogenic FGFR3-TACC3 Fusion Genes in FFPE Samples.

Masahiro KurobeTakahiro KojimaKouichi NishimuraShuya KandoriTakashi KawaharaTakayuki YoshinoSatoshi UenoYuichi IizumiKoji MitsuzukaYoichi AraiHiroshi TsurutaTomonori HabuchiTakashi KobayashiYoshiyuki MatsuiOsamu OgawaMikio SugimotoYoshiyuki KakehiYoshiyuki NagumoMasakazu TsutsumiTakehiro OikawaKoji KikuchiHiroyuki Nishiyama

Published in: PloS one (2016)

We developed an RNA-FISH assay for detection of the FGFR3-TACC3 fusion in FFPE samples of human BC tissues. Screening for not only FGFR3 mutations, but also for FGFR3-TACC3 fusion transcripts has the potential to identify additional patients that can be treated with FGFR inhibitors.

Keyphrases

end stage renal disease
newly diagnosed
high throughput
endothelial cells
ejection fraction
loop mediated isothermal amplification
gene expression
chronic kidney disease
prognostic factors
label free
real time pcr
peritoneal dialysis
transcription factor
risk assessment
climate change
patient reported