Rapid urbanization and growing food demand caused people to be concerned about food safety. Biosensors have gained considerable attention for assessing food safety due to selectivity, and sensitivity but poor stability inherently limits their application. The emergence of machine learning (ML) has enhanced the efficiency of different sensors for food safety assessment. The ML combined with various noninvasive biosensors has been implemented efficiently to monitor food safety by considering the stability of bio-recognition molecules. This review comprehensively summarizes the application of ML-powered biosensors to investigate food safety. Initially, different detector-based biosensors using biological molecules with their advantages and disadvantages and biosensor-related various ML algorithms for food safety monitoring have been discussed. Next, the application of ML-powered biosensors to detect antibiotics, foodborne microorganisms, mycotoxins, pesticides, heavy metals, anions, and persistent organic pollutants has been highlighted for the last five years. The challenges and prospects have also been deliberated. This review provides a new prospect in developing various biosensors for multi-food contaminants powered by suitable ML algorithms to monitor in-situ food safety.