A fluorescent probe based on cyclochalcone for detecting peroxynitrite.
Li-Hao LiuXian-Zhao ShangJian-Hao YuanYi-Ning LuoJia-Yi WangXiao-Lei XueNan JiangKun-Peng WangZhi-Qiang HuPublished in: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (2024)
A novel cyclic chalcone fluorescent probe C-PN was synthesized to detect ONOO - . After reaction with peroxynitrite, the double bond of C-PN in the cyclic chalcone structure was disconnected, which caused the change of intramolecular charge transfer (ICT) effect, emitting blue fluorescence and quenching orange red fluorescence. Visible to the naked eye, the color of the probe solution changed. The probe showed low sensitivity (detection limit = 20.2 nm), short response time (less than 60 s) at low concentration of ONOO - , good visibility, and good selectivity and stability for ONOO - .